DNA Oligonucleotide Synthesis
- length up to 200 bp
- standard scale (40 nmol, 200 nmol and 1 μmol)
- wide range of modifications
- desalted
- possibility of HPLC and PAGE purification
| Fluorofory | Ex. | Em. | Alternativa |
| Cy3TM | 550 | 564 | ATTO 542, DY-530 |
| Cy5TM | 648 | 668 | ATTO 647N |
| FAM | 495 | 520 | DY-490, DY-495 |
| HEX | 538 | 555 | CAL Fluor® Orange 560 |
| JOE | 529 | 555 | CALFluor® Gold 540, Orange 560 |
| ROX | 588 | 608 | ATTO Rho 101 |
| TAMRA | 559 | 583 | CAL Fluor® Red 590, DY-560 |
| TETTM | 522 | 539 | CAL Fluor® Gold 540 |
| Texas Red® | 596 | 613 | CALFluor® Red 610, ATTO Rho101 |
RNA Oligonucleotide Synthesis
- length up to 60 bp
- standard scale (40 nmol, 200 nmol and 1 μmol)
LNA Oligonucleotide Synthesis
Locked nucleic acids (LNA) are modified RNA nucleotides containing a “locked” bicyclic sugar moiety. The bridge between 2' oxygen and 4' carbon “locks” the ribose in the 3'-endo conformation. LNA nucleotides can be mixed with DNA or RNA residues in the oligonucleotide. LNA modified oligos exhibit enhanced hybridization affinity towards complementary DNA or RNA.
Why use LNA?
- finetuning of melting temperature
- enhanced hybridization afinity at shorter length
- improved signal to noise ratio in qPCR assays
- more accurate gene quantification and allelic discrimination
- better single nucleotide mismatch detection
- increased endo- and exonuclease resistance
Applications
- qPCR/PCR applications including multiplex PCR
- gene silencing with antisense LNA oligonucleotides (easy cell entering)
- antisense drug development (non toxic)
- allele discrimination
- microarray analysis
- gene expression analysis
Design Guidelines
- we do not recommend more than 4 consecutive LNA bases
- LNA bases should not be positioned at the 3´end or near the 3´terminus
- try to avoid 3 +G and 3 +C stretches
- GC-content should be between 30-60 %
- larger number of LNA (e.g. 16 and more) increases the risk of self-hybridization
- one LNA base increases Tm by 2-6°C (DNA) and 3-9°C (RNA)
- one LNA positioned at the 5´end will not influence Tm
Ordering information
- LNA is marked in the sequence with + (e.g. +A, +C, +G, +T)
- available in all standard scales (40 nmol, 200 nmol and 1 μmol)
- fully compatible with our wide range of modifications